Philipp J. Keller, PhD

European Molecular Biology Laboratory (EMBL)
Meyerhofstrasse 1
D-69117 Heidelberg

E-Mail: keller@embl.de 
Tel. +49 (0)6221 387 8123
Room 402

Research Projects

In toto reconstruction of zebrafish embryonic development
Publications: Keller et al 2008b, Brown et al 2010 

Evolution of mutational robustness in the yeast genome architecture
Publications: Keller and Knop 2009

Digital Scanned Laser Light Sheet Microscopy (DSLM)
Publications: Keller et al 2008b, Keller and Stelzer 2009

Microtubule dynamic instability and elastic properties in three dimensions
Publications: Keller et al 2008a

Light sheet microscopy-based experimental studies of microtubules
Publications: Keller et al 2006a, Keller et al 2007, Keller et al 2008a

Cell and Developmental Biology applications of Selective Plane Illumination Microscopy
Publications: Keller et al 2006a, Keller et al 2006b, Stelzer and Keller 2007, Pampaloni et al 2007, Keller et al 2007, Keller et al 2008a

Molecular modelling and analysis of yeast spore formation
Publications: Taxis et al 2005, Gordon et al 2006

Peer-Reviewed Primary Publications

Brown K.E., Keller P.J., Ramialison M., Rembold M., Stelzer E.H.K., Loosli F., Wittbrodt, J.
Nlcam modulates midline convergence during anterior neural plate morphogenesis
Developmental Biology, in press, 2010.

Keller P.J., Knop M.
Evolution of mutational robustness in the yeast genome: A link to essential genes and meiotic recombination hotspots
PLoS Genetics, 5(6):e1000533, 2009. PDF

Keller P.J., Schmidt A.D., Wittbrodt J., Stelzer E.H.K.
Reconstruction of zebrafish early embryonic development by Scanned Light Sheet Microscopy
Science, 322:1065-1069, 2008. PDF
Research Highlights in Nature, Nature Cell Biology, Nature Biotechnology, Nature Methods.

Keller P.J., Pampaloni F., Lattanzi G., Stelzer E.H.K.
Three-dimensional microtubule behavior in Xenopus egg extracts reveals four dynamic states and state-dependent elastic properties
Biophysical Journal, 95(3):1474-86, 2008. PDF

Keller P.J., Pampaloni F., Stelzer E.H.K.
Three-dimensional preparation and imaging reveal intrinsic microtubule properties
Nature Methods, 4(10):843-846, 2007. PDF

Gordon O., Taxis C., Keller P.J., Benjak A., Stelzer E.H.K., Simchen G., Knop, M.
Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis
EMBO Journal, 25(16):3856-68, 2006. PDF

Taxis C., Keller P.J., Kavagiou Z., Jensen L.J., Colombelli J., Bork P., Stelzer E.H.K., Knop M.
Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
Journal of Cell Biology, 171:627-640, 2005. PDF

Reviews, Conference Proceedings and Book Chapters

Keller P.J., Stelzer E.H.K.
Digital Scanned Laser Light Sheet Fluorescence Microscopy
In: Live Cell Imaging, Cold Spring Harbor Lab. Press., 2009.

Keller P.J., Stelzer E.H.K.
Light Sheet-Based Fluorescence Microscopes (LSFM, SPIM, DSLM) Reduce Phototoxic Effects by Several Orders of Magnitude
SPIE Conf. Proceed. 7367-60, 2009.

Keller P.J., Schmidt A.D., Wittbrodt J., Stelzer E.H.K.
The Zebrafish Digital Embryo: In toto Reconstruction of Zebrafish Early Embryonic Development with Digital Scanned Laser Light Sheet Fluorescence Microscopy
SPIE Conf. Proceed. 7367-21, 2009.

Keller P.J., Stelzer E.H.K.
Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy
Current Opinion in Neurobiology, 18:624-632, 2009. PDF

Pampaloni F., Keller P.J., Marcello M., Reynaud E.G., Schmidt A., Centanin L., Wittbrodt J., Stelzer, E.H.K.
Live cell imaging in physiologically relevant environments: 3D-squared
Advance Live Cell Imaging, special edition of GIT Imaging & Microscopy, 2008.

Stelzer E.H.K., Keller P.J.
Three-Dimensional Microscopy for a Three-Dimensional World
Innovation, 7:16-8 2007.

Keller, P.J., Stelzer, E.H.K.
Lichtscheiben-Mikroskopie in der molekularen Zellbiophysik
Laborwelt, 5:18-21, 2006.

Keller P.J., Pampaloni F., Stelzer E.H.K.
Life sciences require the third dimension
Current Opinion in Cell Biology, 18(1):117-24, 2006. PDF

Patents

Fully colour-corrected light sheet-based fluorescence microscopy
Inventors: Keller P.J., Stelzer E.H.K.
Application: 22nd August 2008, patent pending

Split beam-scanning microscope illumination system with at least two optical arms
Inventors: Keller P.J., Stelzer E.H.K.
Application: 2nd May 2008, patent pending

Microscope specimen holder
Inventors: Pampaloni F., Keller P.J., Reynaud E.G., Swoger J., Stelzer E.H.K.
International Patent Registry: PCT/EP2006/011863
Application: 8th December 2006 WIPO record

Invited Talks and Conference Contributions

Invited Talk
Shedding light on the system: Reconstructing development with light sheet microscopy.
Trilateral Workshop, Dresden, Germany (2009)

Invited Talk
Shedding light on the system: Reconstructing development with light sheet microscopy.
3rd Mechanobiology Workshop, Singapore (2009)

Seminar
Reconstructing embryonic development with light sheet based microscopy: Shedding light on the system.
Janelia Farm/HHMI Recruiting Symposium, Washington, DC, USA (2009)

Seminar
The Zebrafish Digital Embryo: Quantitative reconstruction of vertebrate development with DSLM.
National University of Singapore (NUS) (2009)

Conference Talk
The Zebrafish Digital Embryo: In Toto Reconstruction of Zebrafish Early Embryonic Development with Digital Scanned Laser Light Sheet Fluorescence Microscopy (DSLM).
European Conferences on Biomedical Optics (ECBO), "Advanced Microscopy Techniques", Munich/Germany (2009)

Invited Talk
The Virtual Embryo: Automated reconstruction of vertebrate development.
9th NIBB-EMBL Meeting, Functional Imaging from Atoms to Organisms, Okazaki/Japan (2009)

Seminar
Quantitative reconstruction of development in entire zebrafish embryos.
University of Dundee, Wellcome Trust Biocentre, Dundee/UK (2009)

Seminar  
Quantitative reconstruction of development in entire zebrafish embryos.
Technical University of Munich, Institute of Neurosciences, Munich/Germany (2009)

Seminar 
Quantitative reconstruction of development in entire Danio rerio embryos.
University of Innsbruck, Center for Molecular Biosciences, Innsbruck/Austria (2009)

Gueat Lecture
The Virtual Embryo.
European Learning Laboratory for the Life Sciences (ELLS), Heidelberg/Germany (2009)

Seminar 
Quantitative reconstruction of development in entire Danio rerio embryos.
University of Chicago, Institute for Genomics and Systems Biology, Chicago/USA (2009)

Seminar 
Quantitative reconstruction of development in entire Danio rerio embryos.
Memorial Sloan-Kettering Cancer Center, New York/USA (2009)

Seminar 
Quantitative reconstruction of development in entire Danio rerio embryos.
The Cancer Research UK London Research Institute, London/UK (2009)

Seminar 
In toto reconstruction of Danio rerio embryonic development.
National Institue for Basic Biology (NIBB), Okazaki/Japan (2008)

Invited Talk
Light Sheet Fluorescence Microscopy. 
International School of Biophysics, Erice/Sicily (2008)

Invited Talk
Reconstruction of Danio rerio embryonic development with DSLM.
Multidimensional Optical Fluorescence Microscopy towards Nanoscopy, Erice/Sicily (2008)

Invited Talk 
In toto reconstruction of Danio rerio embryogenesis with Digital Scanned Laser Light Sheet Microscopy.
INSERM Workshop, Saint-Raphael/France (2008)

Seminar 
In toto reconstruction of Danio rerio embryogenesis with Digital Scanned Laser Light Sheet Microscopy.
École Normale Supérieur (ENS), Paris/France (2008)

Poster 
Investigation of vertebrate embryonic development by DSLM.
Janelia Farm Research Campus, Novel Approaches to Bioimaging, Washington/USA (2008)

Guest Lecture
Cell Lineaging with Scanned Light Sheet Microscopy.
European Learning Laboratory for the Life Sciences (ELLS), Heidelberg/Germany (2007)

Course Instructor
Digital Scanned Laser Light Sheet Microscopy.
EMBO Course: Imaging in 3D and the F-techniques: FRET, FCS, FLIM and FRAP, Singapore (2007)

Conference Talk 
Digital Scanned Light Sheet Microscopy.
Focus on Microscopy, Valencia/Spain (2007)

Conference Talk 
Mutational robustness of sexually structured genomes.
Triangle Meeting, Paris/France (2007)

Lecture
Single Plane Illumination Microscopy and Digital Scanned Laser Light Sheet Microscopy.
EMBL Microscopy Workshop, Heidelberg/Germany (2007)

Lecture
An example of biophysics at EMBL: Cell lineaging with Scanned Light Sheet Microscopy.
International Summer School, Heidelberg/Germany (2007)

Invited Talk
Single Plane Illumination Microscopy in Cell and Developmental Biology.
Microscopy Workshop, The Netherlands Cancer Institute, Amsterdam/Netherlands (2006)

Invited Talk 
Imaging and analysing microtubule dynamic instability in 3D.
CMCI Seminar, Heidelberg/Germany (2006)

Conference Talk 
Microtubule dynamic instability analyzed in three dimensions over time with SPIM.
Meeting of the German Physical Society, Dresden/Germany (2006)

Poster 
3D analysis of microtubule dynamic instability in experiment and in theory.
2nd NIBB-EMBL Meeting, Okazaki/Japan (2006)

Poster 
3D structure of centrosomes and influence on angular distribution of microtubules.
EMBO Conference on Centrosomes and Spindle Pole Bodies, Heidelberg/Germany (2005)

Poster 
3D microtubules dynamic instability analyzed with SPIM. 
FEBS Course and ELMI Meeting on Advanced Light Microscopy, Semmering/Austria (2005)
FEBS/ELMI Award for the Best Poster

Conference Talk 
Cytoskeletal filaments dynamics analyzed in 3D with the Selective Plane Illumination Microscope.
Focus on Microscopy, Jena/Germany (2005)

Research Support

PhD salary: EMBL Internal PhD Fellowship, PIs: Dr. Ernst H.K. Stelzer, Dr. Joachim Wittbrodt, Dr. Michael Knop
Instrumentation: FP6-STREP, 2006-2008, PI: Prof.  Joachim Wittbrodt, volume 140.000 € p.a.